| 质粒名称: | pEP4 E02S EN2L |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20922 |
| 存储实验室: | James Thomson |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 10500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 染色体外/EBNA |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Oct4 and Sox2; Nanog and Lin28 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4900 bp |
| 基因突变: | -- |
| GenBank编号 | NM_002701 NM_024674 NM_024865 NM_003106 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | multiple（如果已销毁则未知） |
| 3' 克隆位点: | multiple（如果已销毁则未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEP4 E02S EN2L was a gift from James Thomson (Addgene plasmid # 20922 ; http://n2t.net/addgene:20922 ; RRID:Addgene_20922) |
| 参考文献部分: | Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEP4 E02S EN2L 15415 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION used in the derivation of human iPS cells using non-integrating
episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28.
ACCESSION .
VERSION .
KEYWORDS pEP4 E02S EN2L
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15415)
AUTHORS Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
TITLE Human Induced Pluripotent Stem Cells Free of Vector and Transgene
Sequences
JOURNAL Science. 2009 Mar 26.
PUBMED 19325077
REFERENCE 2 (bases 1 to 15415)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..15415
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 13..1175
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 244..1166
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
misc_feature 2385..2958
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 2408..2960
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
polyA_signal 3977..4111
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 4641..5803
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 4872..5794
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
misc_feature 6847..7420
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 6870..7422
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
polyA_signal 8116..8250
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 8981..10770
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(11072..12997)
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
primer_bind complement(13436..13454)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 13522..13626
/gene="bla"
/label=AmpR promoter
CDS 13627..14487
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(13845..13864)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 14658..15246
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 15147..15166
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt gggcccgcgg
601 ccggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
1081 cgttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
1501 gacctctcag cctgagggtg aagcangagt cggggtggag agcaactccg atggggcctc
1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
4321 gcccgtcagg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgaccaat
4381 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
4441 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
4501 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
4561 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
4621 attattgact agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
4681 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
4741 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
4801 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
4861 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
4921 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
4981 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
5041 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
5101 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
5161 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
5221 gcccgcggcc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
5281 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
5341 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
5401 accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg
5461 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
5521 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
5581 cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
5641 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
5701 gtaattctcg ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
5761 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacacgtg gtcgcggcca
5821 attctgcagt cgagctcaag cttcgaacta gggccgcgac tctagtgatt catgagtgtg
5881 gatccagctt gtccccaaag cttgccttgc tttgaagcat ccgactgtaa agaatcttca
5941 cctatgcctg tgatttgtgg gcctgaagaa aactatccat ccttgcaaat gtcttctgct
6001 gagatgcctc acacggagac tgtctctcct cttccttcct ccatggatct gcttattcag
6061 gacagccctg attcttccac cagtcccaaa ggcaaacaac ccacttctgc agagaagagt
6121 gtcgcaaaaa aggaagacaa ggtcccggtc aagaaacaga agaccagaac tgtgttctct
6181 tccacccagc tgtgtgtact caatgataga tttcagagac agaaatacct cagcctccag
6241 cagatgcaag aactctccaa catcctgaac ctcagctaca aacaggtgaa gacctggttc
6301 cagaaccaga gaatgaaatc taagaggtgg cagaaaaaca actggccgaa gaatagcaat
6361 ggtgtgacgc agaaggcctc agcacctacc taccccagcc tttactcttc ctaccaccag
6421 ggatgcctgg tgaacccgac tgggaacctt ccaatgtgga gcaaccagac ctggaacaat
6481 tcaacctgga gcaaccagac ccagaacatc cagtcctgga gcaaccactc ctggaacact
6541 cagacctggt gcacccaatc ctggaacaat caggcctgga acagtccctt ctataactgt
6601 ggagaggaat ctctgcagtc ctgcatgcag ttccagccaa attctcctgc cagtgacttg
6661 gaggctgcct tggaagctgc tggggaaggc cttaatgtaa tacagcagac cactaggtat
6721 tttagtactc cacaaaccat ggatttattc ctaaactact ccatgaacat gcaacctgaa
6781 gacgtgtgaa gatgaatcga attcccgcgg ccgctttctc gaggccatcg atgtttaaac
6841 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
6901 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
6961 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
7021 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
7081 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
7141 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
7201 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
7261 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
7321 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
7381 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca ccgcgatcgc
7441 gaattcgatt atcgccatgg gctccgtgtc caaccagcag tttgcaggtg gctgcgccaa
7501 ggcggcagaa gaggcgcccg aggaggcgcc ggaggacgcg gcccgggcgg cggacgagcc
7561 tcagctgctg cacggtgcgg gcatctgtaa gtggttcaac gtgcgcatgg ggttcggctt
7621 cctgtccatg accgcccgcg ccggggtcgc gctcgacccc ccagtggatg tctttgtgca
7681 ccagagtaag ctgcacatgg aagggttccg gagcttgaag gagggtgagg cagtggagtt
7741 cacctttaag aagtcagcca agggtctgga atccatccgt gtcaccggac ctggtggagt
7801 attctgtatt gggagtgaga ggcggccaaa aggaaagagc atgcagaagc gcagatcaaa
7861 aggagacagg tgctacaact gtggaggtct agatcatcat gccaaggaat gcaagctgcc
7921 accccagccc aagaagtgcc acttctgcca gagcatcagc catatggtag cctcatgtcc
7981 gctgaaggcc cagcagggcc ctagtgcaca gggaaagcca acctactttc gagaggaaga
8041 agaagaaatc cacagcccta ccctgctccc ggaggcacag aattgagttt aaacnaatca
8101 ctagctagtt cgaaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
8161 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
8221 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
8281 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
8341 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
8401 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
8461 cccgtcaggc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg tcgatcgact
8521 ctagctagag gatcgatgcc ccgccccgga cgaactaaac ctgactacga catctctgcc
8581 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
8641 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
8701 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
8761 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
8821 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
8881 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
8941 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
9001 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
9061 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
9121 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
9181 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
9241 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
9301 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
9361 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
9421 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
9481 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
9541 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
9601 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
9661 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
9721 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
9781 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
9841 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
9901 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
9961 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
10021 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
10081 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
10141 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
10201 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
10261 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
10321 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
10381 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
10441 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
10501 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
10561 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
10621 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat
10681 gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg
10741 cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa
10801 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
10861 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
10921 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
10981 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
11041 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
11101 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
11161 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
11221 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
11281 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
11341 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
11401 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
11461 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
11521 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
11581 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
11641 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
11701 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
11761 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
11821 acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac
11881 ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc
11941 ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc
12001 ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg
12061 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
12121 ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
12181 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
12241 cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
12301 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg
12361 cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
12421 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
12481 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
12541 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
12601 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc
12661 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
12721 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
12781 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
12841 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
12901 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
12961 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
13021 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
13081 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
13141 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
13201 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
13261 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
13321 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
13381 cagcttatcg atgataagct gtcaaacatg agaattaatt cttgaagacg aaagggcctc
13441 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
13501 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
13561 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
13621 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
13681 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
13741 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
13801 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
13861 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
13921 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
13981 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
14041 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
14101 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
14161 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
14221 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
14281 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
14341 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
14401 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
14461 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
14521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
14581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
14641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
14701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
14761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
14821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
14881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
14941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
15001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
15061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
15121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
15181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
15241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc
15301 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
15361 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcg
//
